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Invitrogen
Phospho-MDMX (Ser367) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-MDMX (Ser367) Antibody (PA5-105383) in ICC/IF
Immunocytochemistry analysis of Phospho-MDMX (Ser367) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-MDMX (Ser367) polyclonal antibody (Product # PA5-105383) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More
Product Details
PA5-105383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MDM4(Accession O15151), corresponding to amino acid residues around phosphorylated Ser367.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of MDM4 only when phosphorylated at Ser367.
Target Information
The MDM2 protein is the primary regulator of p53 protein stability. MDMX is an MDM2-related protein that inhibits MDM2-mediated degradation of p53 via distinct associations with MDM2. The gene that encodes MDMX (also designated MDM4) is a target for amplification in malignant gliomas. ARF interacts with MDMX to sequester MDMX within the nucleolus. This sequestration of MDMX by ARF results in an increase in p53 transactivation. In addition, expression of MDMX can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation. Like MDM2, MDMX also binds p73 and stabilizes the level of p73. Therefore, in striking contrast to p53, the half-life of p73 is increased by binding to MDM2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781B1423; double minute 4 homolog; Double minute 4 protein; double minute 4, human homolog of; p53-binding protein; human homolog of; Mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; MGC132766; p53-binding protein Mdm4; Protein Mdm4; Protein Mdmx; RP11-430C7.1; transformed mouse 3T3 cell double minute 4
Gene Aliases: 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; HDMX; MDM4; MDMX; MRP1
UniProt ID: (Human) O15151, (Rat) Q5XIN1, (Mouse) O35618
Entrez Gene ID: (Human) 4194, (Rat) 304798, (Mouse) 17248
negative regulation of transcription from RNA polymerase II promoter
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell proliferation
negative regulation of cell proliferation
protein ubiquitination
DNA damage response, signal transduction by p53 class mediator
negative regulation of protein catabolic process
negative regulation of apoptotic process
G0 to G1 transition
protein stabilization
negative regulation of cell cycle arrest
cellular response to hypoxia
regulation of signal transduction by p53 class mediator
biological_process
positive regulation of cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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