Invitrogen

Phospho-Profilin 1 (Tyr129) Polyclonal Antibody

Name: Phospho-Profilin 1 (Tyr129) Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143753

查看其他20个Profilin 1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Phospho-Profilin 1 (Tyr129) Antibody in Western Blot (WB)

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Phospho-Profilin 1 (Tyr129) Antibody (PA5-143753) in WB

Western blot of Phospho-Profilin 1 (Tyr129) in HUVEC stimulated with Pervanadate (1 mM) for 30 minutes (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or Phospho-Profilin 1 (Tyr129) polyclonal antibody (Product # PA5-143753) (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & ... View More

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产品信息

PA5-143753

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Phospho-Profilin (Tyr129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr129 in human Profilin-1. This sequence is conserved in rat and mouse Profilin-1. The tyrosine site has some homology to the conserved site (Tyr129) in Profilin-2a and Profilin-2b, the site is not conserved in Profiin-3 and Profilin-4.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

~1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2942981

产品详细信息

This antibody was cross-adsorbed to unphosphorylated Profilin (Tyr-129) then affinity purified using Profilin (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin-1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.

靶标信息

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。