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Invitrogen
Phospho-RSK1 (Thr359, Ser363) Polyclonal Antibody
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Phospho-RSK1 (Thr359, Ser363) Antibody (PA5-38309) in ICC/IF
Immunofluorescence analysis of Phospho-RSK1 (Thr359, Ser363) was performed using 70% confluent log phase HeLa cells treated with TNF-alpha (25 ng/mL, 20 minutes) post overnight serum starvation. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-RSK1 (T... View More
产品信息
PA5-38309
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphopeptide derived from human P90RSK around the phosphorylation site of Thr359/Ser363 (TP-P-K-D-SP)
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
靶标信息
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase that is involved in the MAPK cascade. It is thought to control cellular proliferation and differentiation through the phosphorylation of transcription factors. RPS6KA1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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