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Invitrogen
Phospho-YBX1 (Ser102) Polyclonal Antibody
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Phospho-YBX1 (Ser102) Antibody (PA5-121354) in WB
Western Blot analysis of Phospho-YBX1 (Ser102) in HepG2 cells using a Phospho-YBX1 (Ser102) Polyclonal antibody (Product # PA5-121354) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performe... View More
产品信息
PA5-121354
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic phosphorylated peptide around S102 of human YB-1/YBX1 (NP_004550.2).
偶联物
形式
Liquid
浓度
0.97 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
Positive test controls include: HepG2. The target is usually found in the following locations: Cytoplasm, Cytoplasmic granule, Nucleus, Secreted.
靶标信息
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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