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Invitrogen
Phospho-c-Raf (Ser621) Polyclonal Antibody
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Phospho-c-Raf (Ser621) Antibody (PA1-26655) in WB
Western blot antibody-peptide competition and mutant analysis using a Phospho-c-Raf pSer621 polyclonal antibody (Product # PA1-26655). Immunoprecipitates of HEK293 cells overexpressing wild-type c-Raf and stimulated with 50 ng/ml EGF for 3 minutes (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer ov... View More
产品信息
PA1-26655
产品规格
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
产品详细信息
PA1-26655 detects c-Raf (phospho S621) from human samples. PA1-26655 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology.
PA1-26655 has been successfully used in Western blot procedures.
The PA1-26655 immunogen is a synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.
靶标信息
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
