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RAB27B Antibody (PA5-54096) in ICC/IF
Immunofluorescence of RAB27B was performed using U-87 MG WT and RAB27B KD cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KD cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were sta... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-54096
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.2 mg/mL
规格
20 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: FETSAATGQN VEKAVETLLD LIMKRMEQCV EKTQIPDTVN GGNSGNLDGE KPPEKKCIC
Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.
靶标信息
RAB27B may be involved in targeting uroplakins to urothelial apical membranes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
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