Invitrogen

RANGRF Polyclonal Antibody

查看其他4个RANGRF抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

RANGRF Antibody in Immunocytochemistry (ICC/IF)

RANGRF Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

RANGRF Antibody (PA5-118236) in ICC/IF

Immunofluorescence staining of RANGRF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RANGRF Polyclonal Antibody (Product # PA5-118236, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

RANGRF Antibody in Immunocytochemistry (ICC/IF)

RANGRF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RANGRF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RANGRF Antibody in Western Blot (WB)

RANGRF Antibody in Immunoprecipitation (IP)

Product Image Gallery

RANGRF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of RANGRF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RANGRF Polyclonal Antibody (Product # PA5-118236, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

RANGRF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human RANGRF in human testis with RANGRF Polyclonal Antibody (Product # PA5-118236, 1:500 dilution, formalin-fixed paraffin embedded sections).

RANGRF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human RANGRF in human kidney with RANGRF Polyclonal Antibody (Product # PA5-118236, 1:500 dilution, formalin-fixed paraffin embedded sections).

RANGRF Antibody in Western Blot (WB) — Western Blot using RANGRF Polyclonal Antibody (Product # PA5-118236) at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate, Lane B: MOLT4 Whole Cell Lysate, Lane C: Daudi Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 24 kDa.

RANGRF Antibody in Immunoprecipitation (IP) — RANGRF Immunoprecipitation using: Lane A: 0.5 mg Daudi Whole Cell Lysate 4 µL with RANGRF Polyclonal Antibody (Product # PA5-118236) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: RANGRF Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.

产品信息

PA5-118236

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human RANGRF fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

May regulate the intracellular trafficking of RAN. In cardiac cells seems to regulate the cell surface localization of SCN5A.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MOG1 homolog; Ran guanine nucleotide release factor; Ran-binding protein MOG1; RanGNRF

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基因别名: HSPC165; HSPC236; MDS5; MOG1; RANGNRF; RANGRF

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UniProt ID: (Human) Q9HD47

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Entrez Gene ID: (Human) 29098

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guanyl-nucleotide exchange factor activity Ran guanyl-nucleotide exchange factor activity Ran GTPase binding protein transporter activity sodium channel regulator activity ion channel binding

regulation of heart rate regulation of membrane depolarization ER to Golgi vesicle-mediated transport protein exit from endoplasmic reticulum regulation of membrane potential positive regulation of GTPase activity positive regulation of establishment of protein localization to plasma membrane regulation of membrane depolarization during cardiac muscle cell action potential regulation of sodium ion transmembrane transport positive regulation of protein localization to cell surface regulation of sodium ion transmembrane transporter activity