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Invitrogen
RET Monoclonal Antibody (421R25)
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FIGURE: 1 / 4
RET Antibody (AHO1482) in WB
Western blot was performed using Anti-RET Monoclonal Antibody (421R25) (Product # AHO1482) and 110-180kDa bands corresponding to RET were observed in SH-SY5Y, SK-N-AS, Neuro-2a and Rat Cerebellum. No bands were observed in HeLa, Hep G2, HEK-293, Rat Liver and Rat Kidney. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SK-N-AS (Lane 2), HeLa (Lane 3), Hep G2 (Lane 4), HEK-293 (Lane 5), Neuro-2a (Lane 6), Rat Cerebellum (Lane 7), Rat Liver (Lane 8) and Rat Kidney (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Prote... View More
Product Details
AHO1482
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
421R25
Immunogen
Recombinant fragment of human c-Ret extracellular domain expressed in E. coli.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The receptor tyrosine kinase RET is a member of the cadherin superfamily. RET plays a crucial role in neural crest development, and can undergo oncogenic activation by cytogenetic rearrangement. RET transduce signals for cell growth and differentiation. Mutations in the RET gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for the RET gene. Additional transcript variants have been described for RET but their biological validity has not been confirmed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cadherin family member 12; Cadherin family member 12 (CDHF12); Cadherin related family member 16 (CDHR16); cadherin-related family member 16; CUX1/RET fusion; EC 2.7.10.1; ELKS; Hydroxyaryl protein kinase; hydroxyaryl-protein kinase; kinase Ret; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; OTTHUMP00000216967; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor Ret; rearranged during transfection; receptor tyrosine kinase; RET ELE1; Ret gene for receptor tyrosin; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease); Ret proto-oncogene (multiple endocrine neoplasia MEN2A MEN2B and medullary thyroid carcinoma 1 Hirschsprung disease); RET receptor tyrosine kinase; RET transforming sequence
Gene Aliases: c-Ret; CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET; RET-ELE1; RET51; RET9
UniProt ID: (Human) P07949, (Mouse) P35546, (Rat) G3V9H8
Entrez Gene ID: (Human) 5979, (Mouse) 19713, (Rat) 24716
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor activity
Ras guanyl-nucleotide exchange factor activity
calcium ion binding
protein binding
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
molecular_function
transmembrane signal receptor
MAPK cascade
ureteric bud development
neural crest cell migration
embryonic epithelial tube formation
protein phosphorylation
homophilic cell adhesion via plasma membrane adhesion molecules
neuron cell-cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
posterior midgut development
positive regulation of neuron projection development
positive regulation of neuron maturation
peptidyl-tyrosine phosphorylation
regulation of cell adhesion
positive regulation of cell migration
membrane protein proteolysis
positive regulation of cell adhesion mediated by integrin
ureter maturation
response to drug
neuron maturation
positive regulation of GTPase activity
positive regulation of cell size
positive regulation of transcription, DNA-templated
response to pain
enteric nervous system development
regulation of axonogenesis
retina development in camera-type eye
innervation
Peyer's patch morphogenesis
cellular response to retinoic acid
positive regulation of metanephric glomerulus development
lymphocyte migration into lymphoid organs
positive regulation of serine phosphorylation of STAT3 protein
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
nervous system development
anatomical structure morphogenesis
positive regulation of gene expression
phosphorylation
neuron differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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