Invitrogen

RFC1 Polyclonal Antibody

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说明书
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问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
RFC1 Polyclonal Antibody

产品信息

PA5-79923

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL
-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL
-

免疫细胞化学 (ICC/IF)

5 µg/mL
-

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1 (46-64aa NSSRKEDDFKQKQPSKKKR).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.01mg sodium azide, 0.05mg thimerosal

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2747038

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human 293T whole cell, human Hela whole cell, human HepG2 whole cell, human Raji whole cell, human K562 whole cell, human CACO-2 whole cell. IHC: human mammary cancer tissue. ICC/IF: U2OS cell. Flow: Hela cell.

靶标信息

Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: A1 140 kDa subunit; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; DNA-binding protein PO-GA; MHC binding factor, beta; replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; Replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit

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基因别名: A1; MHCBFB; PO-GA; RECC1; RFC; RFC1; RFC140

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UniProt ID: (Human) P35251

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Entrez Gene ID: (Human) 5981

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