Invitrogen

RPA70 Monoclonal Antibody (11H4)

Name: RPA70 Monoclonal Antibody (11H4)
Brand: Invitrogen
SKU: MA5-36226

查看其他20个RPA70抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (6)

RPA70 Antibody in Immunocytochemistry (ICC/IF)

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RPA70 Antibody (MA5-36226) in ICC/IF

Immunocytochemistry analysis of RPA70 in A549 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in RPA70 monoclonal antibody (Product # MA5-36226) at a dilution of 2 µg/mL (overnight, 4°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C).

RPA70 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen Image RPA70 Monoclonal Antibody (11H4)

产品信息

MA5-36226

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

11H4

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human RPA70 (533-568aa QESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFR).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884060

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: HELA whole cell, MCF-7 whole cell, K562 whole cell, COS-7 whole cell, Caco-2 whole cell, A549 whole cell, HEPG2 whole cell, PC-3 whole cell, HEPA1-6 whole cell. IHC: human intestinal cancer tissue, human lung cancer tissue, human lung cancer tissue. ICC/IF: A549 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: MSTP075; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1, 70kDa; RF-A protein 1; RP-A p70; Single-stranded DNA-binding protein

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基因别名: 5031405K23Rik; 70kDa; AA589576; AW557552; HSSB; MST075; REPA1; RF-A; RP-A; Rpa; RPA1; RPA70

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UniProt ID: (Human) A8K0Y9, (Mouse) Q8VEE4

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Entrez Gene ID: (Human) 6117, (Mouse) 68275

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功能

damaged DNA binding single-stranded DNA binding protein binding metal ion binding nucleic acid binding DNA binding chromatin binding DNA metabolism protein

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