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Invitrogen
RPS27L Polyclonal Antibody
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RPS27L Antibody (PA5-90743) in WB
Western blot analysis of RPS27L in extracts of various cell lines. Samples were incubated with RPS27L Polyclonal antibody (Product # PA5-90743) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 2s.
产品信息
PA5-90743
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-84 of human RPS27L (NP_0570041)
偶联物
形式
Liquid
浓度
0.61 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MPLARDLLHP SLEEEKKKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH; Positive Samples: 293T, HepG2, SH-SY5Y, Mouse liver, Mouse pancreas, Rat heart, Rat liver
靶标信息
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; OTTHUMP00000200786; OTTHUMP00000201692; Ribosomal protein eS27-like; ribosomal protein S27; ribosomal protein S27-like; RPS27L; Small ribosomal subunit protein eS27-like
基因别名: 1810034D23Rik; RPS27L
UniProt ID: (Human) Q71UM5, (Rat) P24051, (Mouse) Q6ZWY3
Entrez Gene ID: (Human) 51065, (Rat) 681429, (Mouse) 67941
ribosomal small subunit assembly
DNA repair
rRNA processing
translation
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
mitotic G1 DNA damage checkpoint
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of translation
