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Invitrogen
RSK3 Polyclonal Antibody
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RSK3 Antibody (PA5-19697) in ICC/IF
Immunofluorescent staining of HeLa cells using PA5-19697, anti-RSK3 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with PBS-T (20mins), BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain ... View More
产品信息
PA5-19697
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RSK3.
偶联物
形式
Liquid
浓度
0.8 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
PA5-19697 targets RSK3 in IF and WB applications and shows reactivity with Human and mouse samples.
The PA5-19697 immunogen is synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RSK3.
PA5-19697 detects RSK3 which has a predicted molecular weight of approximately 110 kDa.
靶标信息
RPS6KA2 (RSK3) serine/threonine kinase is a member of the ribosomal S6 kinase (RSK) family. It is believed that RPS6KA2 is a signal-transducing intermediate in the cellular response to several growth factors. It is found in many tissues, but in higher levels in lung and skeletal muscle. The p90 ribosomal S6 kinases (RSK)1-4 are downstream members of the extracellular signal-regulated kinase (ERK)/MAPK cascade. The loss of RSK2 activity in humans leads to Coffin-Lowry syndrome, which is characterized by mental retardation and growth deficits and may have a tonic regulation on G-protein coupled signaling.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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