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Invitrogen
RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC), Alexa Fluor™ Plus 647
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RUNX1 Antibody (740114TP647) in Flow
Flow cytometry analysis of RUNX1 Recombinant Monoclonal Rat Antibody (RXDMC), Alexa Fluor™ Plus 647 (Product # 740114TP647) was performed using Jurkat (log phase) and IMR-32 cells. Cells were fixed with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained with 1 µg of the RUNX1 Recombinant Monoclonal Rat Antibody (RXDMC), Alexa Fluor™ Plus 647 (Product # 740114TP647). RUNX1 higher expression was observed in Jurkat (log phase) (pink histogram) in comparison to IMR-32 (blue histogram).... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740114TP647
产品规格
种属反应
Human
宿主/亚型
Rat
/ IgG2a, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
RXDMC
偶联物
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
Alexa Fluor™ Plus 647
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; CBF-alpha 2; CBF-alpha-2; Core-binding factor subunit alpha-2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
基因别名: AML1; CBFA2; RUNX1
Entrez Gene ID: (Human) 861
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
