Invitrogen

Rab4 Polyclonal Antibody

Name: Rab4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA3-912
Price: ... USD
Availability: InStock

高级验证

5个已发表图片

13 篇参考文献

查看其他10个Rab4抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:

{{$ctrl.translations[$ctrl.isSkuNotesCollapsed ? 'antibody.pdp.skuNotes.viewMore' : 'antibody.pdp.skuNotes.viewLess']}}

Rab4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 17

Rab4 Antibody (PA3-912) in ICC/IF

Immunofluorescence analysis of RAB4A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB4A Rabbit polyclonal Antibody (Product # PA3-912) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

Rab4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rab4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Rab4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RAB4A specific validated siRNA (Silencer® select Product # s11675) and decrease in signal intensity was observed in western blot application using Anti-RAB4A Rabbit Polyclonal Antibody (Product # PA3-912). {KD}

产品信息

PA3-912

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

1:200

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:250

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat

已发表种属

Bovine, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Purified, recombinant human Rab4 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

whole serum

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2269382

产品详细信息

PA3-912 detects Rab4 from human and mouse samples. This antibody does not detect other Rab isoforms (Rab1, Rab3, Rab5, or Rab6).

PA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~24 kDa band representing Rab4 from transfected HeLa cells.

The PA3-912 immunogen is purified recombinant human Rab4 protein.