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Invitrogen
Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 647
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Mouse IgG (H+L) Secondary Antibody (SA5-10391-AF647) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 647 (Product # SA5-10391-AF647) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer at room temperature and stained with beta Tubulin Monoclonal Antibody (2 28 33) (Product # 32-2600, 1:500) in SuperBlock™ Blocking Buffer (Product # 37515, 1:20) overni... View More
产品信息
SA5-10391-AF647
应用
建议稀释比
免疫组化（石蜡） (IHC (P))
1:500
免疫细胞化学 (ICC/IF)
1:1,000-1:4,000
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Secondary Antibody
抗原
Purified Mouse IgG
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
靶标
IgG
抗体形式
Whole Antibody
产品详细信息
Rabbit anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 647 can detect both Fab2 and Fc region of Mouse IgG. It can also detect all subtypes of Mouse IgG (Mouse IgG1,2a,2b and 3). It has <20% cross reactivity to Human IgG and Rat IgM. It showed minimum to no cross-reactivity to various species immunoglobulins tested.
This recombinant antibody has been engineered with point mutations that eliminate binding to Fc receptors, enabling reduction in background and superior signal to noise.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
Protein conjugates made with Alexa Fluor™ dyes produce fluorescence output that surpasses that of other spectrally similar fluorophore-labeled proteins. Alexa Fluor™ dyes are available in a wide selection of fluorescent colors and are more photostable than most other dyes. Photostability is a key characteristic of Alexa Fluor dye™ conjugates, allowing more time for image capture. In addition to their exceptional brightness and the wide selection of dyes across the spectrum, you'll get photostability you can rely on with Alexa Fluor™ dyes and dye conjugates.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
