Rockland

Rad9 S1129 (pan reactive) Polyclonal Antibody

Name: Rad9 S1129 (pan reactive) Polyclonal Antibody
Brand: Rockland
SKU: 600-401-346

查看其他22个RAD9抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Rad9 S1129 (pan reactive) Antibody in Western Blot (WB)

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Rad9 S1129 (pan reactive) Antibody (600-401-346) in WB

Affinity purified antibody to yeast Rad9 (1125-1139 pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphoryla... View More

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Immunogen image

Immunogen Image Rad9 S1129 (pan reactive) Polyclonal Antibody

产品信息

600-401-346

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1:5,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1120-1145 from the aa1309 yeast Rad9 protein conjugated to KLH.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against an internal sequence of yeast Rad9 at the S1129 residue. This pan reactive polyclonal antibody reacts equally with both phosphorylated and non- phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown.

靶标信息

RAD9 is a component of the 9-1-1 cell cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesions upon damage by the RAD17-replication factor C clamp loader complex. RAD9 acts as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair.

仅用于科研。不用于诊断过程。未经明确授权不得转售。