Invitrogen

RyR1 Monoclonal Antibody (34C)

Name: RyR1 Monoclonal Antibody (34C)
Brand: Invitrogen
SKU: MA3-925

155 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

RyR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 2

RyR1 Antibody (MA3-925) in IHC (P)

Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal an... View More

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产品信息

MA3-925

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1:1,000

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免疫组织化学（悬浮细胞） (IHC (Free))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看24篇已发表文章 24篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看9篇已发表文章 9篇已发表文章

亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Amphibian, Bovine, Dog, Chicken, Fish, Human, Mustelid, Mouse, Non-human primate, Sheep, Rabbit, Rat

已发表种属

Bovine, C. elegans, Cat, Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

34C

抗原

Partially purified chicken pectoral muscle ryanodine receptor.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2254138

产品详细信息

MA3-925 detects ryanodine receptor (RyR)-1 and RyR-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chickens this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, MA3-925 detects the alpha and beta isoforms and in fish it detects the alpha isoform.

MA3-925 has been successfully used in Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain with MA3-925 results in intense staining of cerebellar Purkinje neurons.

The MA3-925 antigen is partially purified chicken pectoral muscle ryanodine receptor.

靶标信息

Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500 kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: calcium release channel; central core disease of muscle; protein phosphatase 1, regulatory subunit 137; Ryanodine receptor; Ryanodine receptor 1; ryanodine receptor 1 (skeletal); ryanodine receptor 1, skeletal muscle; ryanodine receptor 1-like, skeletal muscle; RYR-1; sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; Type 1 ryanodine receptor; type 1-like ryanodine receptor; unnamed protein product

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基因别名: AI528790; CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; RYR1; Ryr1l; SKRR

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UniProt ID: (Rabbit) P11716, (Human) P21817, (Mouse) E9PZQ0