Search
Invitrogen
SMAD1/SMAD5 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
图: 1 / 2
SMAD1/SMAD5 Antibody (PA5-80036) in WB
Western blot analysis of SMAD1/SMAD5 in Lane 1: rat kidney tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse kidney tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: human HeLa cell lysate, Lane 7: human A375 cell lysate, Lane 8: human HepG2 cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minu... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-80036
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mammal,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human SMAD1/SMAD5 (KRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEK).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat kidney tissue, rat testis tissue, mouse brain tissue, mouse kidney tissue, mouse testis tissue, human Hela cell, human A375 cell, human HepG2 cell.
靶标信息
The Smad family of proteins are functioning in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of a TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 is then able to form a complex with Smad4. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. In mammals, eight Smad proteins have been identified to date. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads (I-Smads). The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-beta and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-Smad binding to Co-Smad, Smad4, and translocation to the nucleus where they regulate TGF-beta target genes. Smad6 and Smad7 belong to the I-Smad which bind to the type I receptor or Smad4 and block their interaction with R-Smads.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BSP-1; Dwarfin-A; Dwarfin-C; Dwf-A; Dwf-C; hSMAD1; hSmad5; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog 5; MAD homolog1 (mothers against decapentaplegic, Drosophila); Mad-related protein 1; mMad1; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against DPP homolog 1; mothers against DPP homolog 5; Mothers-against-DPP-related 1; mSmad5; OTTHUMP00000223331; SMAD; SMAD 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; Transforming growth factor-beta-signaling protein 1
基因别名: 1110051M15Rik; AI451355; AI528653; BSP1; Dwf-C; Mad1; MADH1; MADH5; MADR1; Mlp1; Msmad5; MusMLP; SMAD1; SMAD5
UniProt ID: (Mouse) P70340, (Rat) P97588, (Mouse) P97454, (Rat) Q9R1V3
Entrez Gene ID: (Mouse) 17125, (Rat) 25671, (Mouse) 17129, (Rat) 59328
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein kinase binding
identical protein binding
protein homodimerization activity
metal ion binding
protein heterodimerization activity
co-SMAD binding
I-SMAD binding
ubiquitin protein ligase binding
DNA-binding transcription factor
gene-specific transcriptional regulator
MAPK cascade
mesodermal cell fate commitment
osteoblast fate commitment
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
protein phosphorylation
transforming growth factor beta receptor signaling pathway
SMAD protein complex assembly
gamete generation
negative regulation of cell proliferation
positive regulation of gene expression
BMP signaling pathway
midbrain development
hindbrain development
response to drug
homeostatic process
positive regulation of cell differentiation
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
cartilage development
cardiac muscle cell proliferation
bone development
positive regulation of cartilage development
cellular response to organic cyclic compound
cellular response to BMP stimulus
positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus
ureteric bud development
kidney development
inflammatory response
embryonic pattern specification
response to organonitrogen compound
negative regulation of muscle cell apoptotic process
wound healing
positive regulation of dendrite morphogenesis
angiogenesis
Mullerian duct regression
multicellular organism development
germ cell development
cell differentiation
erythrocyte differentiation
cardiac muscle contraction
ossification
regulation of ossification
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
