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Invitrogen
SMCR7L Polyclonal Antibody
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SMCR7L Antibody (PA5-143958) in ICC/IF
Immunocytochemistry analysis of SMCR7L in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SMCR7L Polyclonal antibody (Product # PA5-143958) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More
产品信息
PA5-143958
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human MIEF1 recombinant protein (Position: S189-T463).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HT1080 whole cell, human Jurkat whole cell, human K562 whole cell, human HL-60 whole cell, human HEK293 whole cell, human U-937 whole cell, human HepG2 whole cell, rat heart tissue, rat kidney tissue, mouse kidney tissue. IHC: human renal clear cell carcinoma tissue, human rectal cancer tissue. ICC/IF: U20S cell. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: mitochondrial dynamic protein MID51; mitochondrial dynamic protein of 51 kDa; mitochondrial dynamic protein of 51 kDa homolog; Mitochondrial dynamics protein MID51; Mitochondrial dynamics protein MIEF1; Mitochondrial dynamics protein of 51 kDa; Mitochondrial dynamics protein of 51 kDa homolog; Mitochondrial elongation factor 1; SMCR7-like protein; Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like; Smith-Magenis syndrome chromosome region, candidate 7-like
基因别名: A230016E22; AI452372; dJ1104E15.3; HSU79252; MID51; MIEF1; SMCR7L
UniProt ID: (Human) Q9NQG6, (Mouse) Q8BGV8, (Rat) Q5XIS8
Entrez Gene ID: (Human) 54471, (Mouse) 239555, (Rat) 315141
