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Invitrogen
SPAG16 Polyclonal Antibody
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SPAG16 Antibody (PA5-142541) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of SPAG16 in A431 cells using SPAG16 Polyclonal Antibody (Product # PA5-142541). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-142541
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence C-KGVTELRTVGNVPD.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Additional Information: This antibody is expected to recognize isoform 1 and 2 (NP_078808.3; NP_001020607.1).
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
靶标信息
Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Pf20 protein homolog; Sperm-associated antigen 16 protein; sperm-associated WD repeat protein; spg16; WD repeat domain 29
基因别名: 4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; PF20; SPAG16; WDR29
UniProt ID: (Human) Q8N0X2, (Mouse) Q8K450
Entrez Gene ID: (Human) 79582, (Mouse) 66722
