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Invitrogen
SPC24 Polyclonal Antibody
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SPC24 Antibody (PA5-95812) in WB
Western blot analysis of SPC24 in HeLa cells. Samples were incubated with SPC24 Polyclonal antibody (Product # PA5-95812) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
产品信息
PA5-95812
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SPC24 (NP_8723191)
偶联物
形式
Liquid
浓度
2.12 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.05% ProClin 300
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS LLQLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW; Positive Samples: Jurkat, HeLa, 293T; Cellular Location: Chromosome, Nucleus, centromere, kinetochore
靶标信息
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: hSpc24; Kinetochore protein Spc24; SPC24, NDC80 kinetochore complex component, homolog; spindle pole body component 24 homolog
基因别名: 2410030K01Rik; AV109292; SPBC24; SPC24
UniProt ID: (Human) Q8NBT2, (Mouse) Q9D083
Entrez Gene ID: (Human) 147841, (Mouse) 67629
