Invitrogen

STS Polyclonal Antibody

Name: STS Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-80083

查看其他11个STS抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

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STS Antibody (PA5-80083) in WB

Western blot analysis of STS in Lane 1: human HeLa whole cell lysate, Lane 2: human placenta tissue lysate, Lane 3: human MCF-7 whole cell lysate, Lane 4: human HepG2 whole cell lysate, Lane 5: human PANC-1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, in... View More

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Immunogen image

Immunogen Image STS Polyclonal Antibody

产品信息

PA5-80083

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human Steroid sulfatase recombinant protein (Position: K297-E396).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2747198

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human HepG2 whole cell, human PANC-1 whole cellFlow: Hela cells.

靶标信息

The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI).

仅用于科研。不用于诊断过程。未经明确授权不得转售。