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Invitrogen
SUP45 Polyclonal Antibody
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SUP45 Antibody (PA5-144559) in WB
Western blot analysis of SUP45 in recombinant protein using a dilution of 1:1,000. Samples were incubated with SUP45 polyclonal antibody (Product # PA5-144559). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa.
产品信息
PA5-144559
产品规格
种属反应
Yeast
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Saccharomyces cerevisiae (strain ATCC 204508/S288c) (Baker's yeast) SUP45 protein (26-262aa)
偶联物
形式
Liquid
浓度
5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% Proclin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed:7556078, PubMed:20947765, PubMed:34413231). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:7556078, PubMed:20947765). SUP45/eRF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed:7556078, PubMed:20947765, PubMed:34413231). Following GTP hydrolysis by SUP35/eRF3, SUP35/eRF3 dissociates, permitting SUP45/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed:7556078, PubMed:20947765, PubMed:34413231).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Omnipotent suppressor protein 1; SAL4; SUP1
基因别名: SAL4; SUP1; SUP45; SUP47; YBR1120; YBR143C
UniProt ID: (Baker's yeast) P12385
Entrez Gene ID: (Baker's yeast) 852440
