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Invitrogen
TCF7 Polyclonal Antibody
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TCF7 Antibody (PA5-77813) in WB
Western blot analysis of TCF7 in human cervical cancer cell line (HeLa) lysate using 10 µg of protein. Sample was prepared with 5% milk and TBST, incubated with TCF7 polyclonal antibody (Product # PA5-77813) using a dilution of 1:1000 (2 hours at RT) and followed by a Goat Anti-Rabbit HRP secondary antibody at a dilution of 1:3000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: Cervical Cancer cell line (HeLa) lysate.
产品信息
PA5-77813
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide from the C-terminal of Human TCF7 (284-384 aa), conjugated to Keyhole Limpet Haemocyanin (KLH)
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
产品详细信息
A 1:1,000 dilution of PA5-77813 was sufficient for detection of TCF7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects 42 kDa, additional band at approximately 54 kDa is another isoform.
靶标信息
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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