Rockland

THEM4 Polyclonal Antibody

Name: THEM4 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-FE0
Price: 7308.00 CNY
Availability: InStock

查看其他7个THEM4抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)

THEM4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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THEM4 Antibody (600-401-FE0) in IHC (P)

Immunohistochemistry of THEM4 antibody. Tissue: Human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: THEM4 antibody at 2.5 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: THEM4 is cytoplasmic. Staining: THEM4 as precipitated brown signal with purple nuclear counterstain.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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产品信息

600-401-FE0

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

2.5 µg/mL

免疫细胞化学 (ICC/IF)

20 µg/mL

酶联免疫吸附实验 (ELISA)

1:10,000-1:20,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-THEM4 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid synthetic peptide near the internal region of human THEM4.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

靶标信息

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

仅用于科研。不用于诊断过程。未经明确授权不得转售。