Invitrogen

TMEM163 Polyclonal Antibody

1 篇参考文献

查看其他3个TMEM163抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

TMEM163 Antibody in Immunocytochemistry (ICC/IF)

TMEM163 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

TMEM163 Antibody (PA5-114329) in ICC/IF

Immunocytochemical analysis of TMEM163 in 293T cells using a TMEM163 polyclonal antibody (Product #PA5-114329). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

TMEM163 Antibody in Immunocytochemistry (ICC/IF)

TMEM163 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TMEM163 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TMEM163 Antibody in Western Blot (WB)

TMEM163 Antibody in Flow Cytometry (Flow)

Product Image Gallery

TMEM163 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of TMEM163 in 293T cells using a TMEM163 polyclonal antibody (Product #PA5-114329). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

TMEM163 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TMEM163 in paraffin-embedded human prostate carcinoma tissue using a polyclonal antibody (Product #PA5-114329). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

TMEM163 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TMEM163 in paraffin-embedded rat large intestine tissue using a polyclonal antibody (Product #PA5-114329). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

TMEM163 Antibody in Western Blot (WB) — Western blot analysis of TMEM163 using a TMEM163 polyclonal antibody (Product #PA5-114329). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:1000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skin tissue lysate, Lane 2: Human small intestine tissue lysate, Lane 3: Human mouse colon tissue lysate predicted band size: 31 kDa observed band size: 38 kDa

TMEM163 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of TMEM163 using F9 cells and a TMEM163 polyclonal antibody (Product #PA5-114329). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunogen image

TMEM163 Polyclonal Antibody

Immunogen Image TMEM163 Polyclonal Antibody

产品信息

PA5-114329

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

流式细胞分析 (Flow)

1:50-1:100

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within Human TMEM163 aa 55-104

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

May bind zinc and other divalent cations and recruit them to vesicular organelles. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Synaptic vesicle membrane protein of 31 kDa; synaptic vesicle protein of 30 kD; Transmembrane protein 163

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基因别名: 2610024A01Rik; DC29; RGD1306212; SV31; TMEM163

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UniProt ID: (Human) Q8TC26, (Rat) A9CMA6, (Mouse) Q8C996

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Entrez Gene ID: (Human) 81615, (Rat) 360839, (Mouse) 72160

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zinc ion binding

biological_process