Agrisera

TOC1-P/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Polyclonal Antibody

Name: TOC1-P\/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Polyclonal Antibody
Brand: Agrisera
SKU: AS22-4701

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

TOC1-P/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Antibody in Western Blot (WB)

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TOC1-P/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Antibody (AS22-4701) in WB

8 µg/well of total protein extracted freshly from 10-day-old Arabidopsis thaliana seedlings with extraction buffer (100 m M Tris·Cl, p H 7.5/150 m M Na Cl/0.5% NP-40/1 m M EDTA/3 m M DTT/1 m M PMSF/2 m M Na F/2 m M Na 3 VO 4/1 µg mL-1 leupeptin/1 µg mL-1 aprotinin/1 µg mL-1 pepstatin/5 µg mL-1 antipain/5 µg mL-1 chymostatin/50 µM MG 132/50 µM MG 115/50 µM ALLN) and denatured at room temperature 24 °C for 3 ... View More

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产品信息

AS22-4701

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Plant

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

KLH-conjugated peptide derived from Arabidopsis thaliana TOC1 phosphorylated protein sequence.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

50 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

For reconstitution add 50 µL of sterile water.

Specific Species Reactivity: Arabidopsis thaliana

靶标信息

TOC1-P encodes a nuclear-localized pseudo-response regulator that functions as a core component of the Arabidopsis circadian oscillator, acting predominantly as a transcriptional repressor within interlocked feedback loops that shape daily rhythms in gene expression and development. The gene produces a multi-exon transcript whose protein product is characterized by an N-terminal receiver-like (pseudo-receiver) domain typical of response regulators but lacking canonical phospho-acceptor activity, and a C-terminal CCT (CONSTANS, CO-like, TOC1) DNA-binding region required for direct promoter occupancy and repression of central clock genes such as CCA1 and LHY; deletion or disruption of the CCT region abolishes DNA binding and impairs repression. TOC1-P activity is further tuned by post-translational regulation, including N-terminal phosphorylation that modulates interactions with specific transcriptional regulators at rhythmic promoters, and protein-protein interactions that influence nuclear accumulation and stability, thereby coupling clock timing to photoperiodic outputs such as growth and flowering and to environmental responses including temperature-gated elongation programs.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: APRR1; AtTOC1; MFB13.13; MFB13_13; PRR1; PSEUDO-RESPONSE REGULATOR 1; TIMING OF CAB EXPRESSION 1

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TOC1-P/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Polyclonal Antibody

TOC1-P/TIMING OF CAB EXPRESSION 1 Arabidopsis clock gene, phosphorylated Antibody (AS22-4701) in WB

产品信息

AS22-4701

免疫印迹 (WB)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

规格

纯化类型

保存液

内含物

保存条件

运输条件

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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