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Invitrogen
TRIF Polyclonal Antibody
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TRIF Antibody (PA1-20824) in ICC/IF
Immunofluorescence analysis of TIR domain-containing adapter molecule 1 was performed using 70% confluent log phase Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIF Polyclonal Antibody (Product # PA1-20824) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Al... View More
产品信息
PA1-20824
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide, corresponding to amino acids 143-158 of Mouse TRIF.
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
By Western blot, PA1-20824 detects the ~ 66 kDa TRIF protein.
靶标信息
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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