Invitrogen

TRIM44 Polyclonal Antibody

查看其他8个TRIM44抗体

问题与答案

问题与答案

问题与答案

Additional Information:

{{banner.description}}

{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

抗体检测数据 (4)

TRIM44 Antibody in Immunocytochemistry (ICC/IF)

TRIM44 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

TRIM44 Antibody (PA5-116073) in ICC/IF

Immunocytochemistry analysis of TRIM44 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116073) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

TRIM44 Antibody in Immunocytochemistry (ICC/IF)

TRIM44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TRIM44 Antibody in Western Blot (WB)

TRIM44 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

TRIM44 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of TRIM44 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116073) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

TRIM44 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of TRIM44 in human liver tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-116073) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.

TRIM44 Antibody in Western Blot (WB) — Western blot analysis of TRIM44 in various samples (Lane 1: rat heart treated with blocking peptide Lane 2: Rat heart; Lane 3: Hepg2). Samples were incubated with polyclonal antibody (Product # PA5-116073).

TRIM44 Antibody in Western Blot (WB) — Western blot analysis of TRIM44 in human liver tissue. Samples were incubated with polyclonal antibody (Product # PA5-116073).

Immunogen image

TRIM44 Polyclonal Antibody

Immunogen Image TRIM44 Polyclonal Antibody

产品信息

PA5-116073

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human TRIM44(Accession Q96DX7), corresponding to amino acid residues E154-I204.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody detects endogenous levels of total TRIM44.

靶标信息

May play a role in the process of differentiation and maturation of neuronal cells. May regulate the activity of TRIM17.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: brain cDNA 7; DIPB; DIPB protein; Protein DIPB; Protein Mc7; tripartite motif protein 44; Tripartite motif-containing protein 44

查看更多收起

基因别名: DIPB; Mc7; TRIM44

查看更多收起

UniProt ID: (Mouse) Q9QXA7, (Rat) Q6QA27

查看更多收起

Entrez Gene ID: (Mouse) 80985, (Rat) 362172

查看更多收起

zinc ion binding metal ion binding ubiquitin-protein ligase