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Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is thyroid or lung carcinoma.
TTF1 is a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。