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产品信息
14-9739-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
7UNA8U6
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 7UNA8U6 reacts with human, mouse, and rat TdT. TdT, or terminal-deoxynucleotidyl transferase, is a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. TdT plays a critical role during TCR and Ig gene rearrangement and functions to increase the diversity of the lymphocyte repertoire through the addition of N-nucleotides onto the ends of newly formed P-nucleotides. After positive selection, expression of TdT becomes decreased or absent. Expression of TdT has been shown to be present in lymphoma or lymphoblastic leukemia cells.
Applications Reported: This 7UNA8U6 antibody has been reported for use in western blotting and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This 7UNA8U6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human and mouse tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. This 7UNA8U6 antibody has also been tested by western blot analysis of reduced cell lysate prepared from mouse and rat cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Terminal Deoxynucleotidyl Transferase (TdT) is a DNA polymerase located in the cell nucleus which catalyses the polymerization of deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide initiators and functions without a template. TdT is considered to be a highly specific marker for the diagnosis and classification of acute lymphoblastic lymphoma/leuksemias. The determination of TdT expression is most valuable when it is different to differentiate histologically between lymphoblastic lymphoma and Burkitt's lymphoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Deoxynucleotidyltransferase terminal; deoxynucleotidyltransferase, terminal; DNA nucleotidylexotransferase; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; nucleosidetriphosphate:DNA deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; terminal deoxynucleotidyl transferase; Terminal deoxynucleotidyltransferase; terminal deoxyribonucleotidyltransferase; Terminal transferase
基因别名: DNTT; TDT
UniProt ID: (Human) P04053, (Mouse) P09838
Entrez Gene ID: (Human) 1791, (Mouse) 21673, (Rat) 294051
