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Invitrogen
UBE2Q2 Polyclonal Antibody
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UBE2Q2 Antibody (PA5-80202) in ICC/IF
Immunocytochemistry analysis of UBE2Q2 using anti-UBE2Q2 antibody (Product # PA5-80202) . UBE2Q2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-UBE2Q2 antibody (Product # PA5-80202) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAP... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-80202
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the N-terminus of human UBE2Q2 (83-123aa LERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVEMLDQ).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, A431 whole cell, MCF-7 whole cell, SW620 whole cell. IHC: human lung cancer tissue. ICC/IF: U20S cell. Flow: A549 cell.
靶标信息
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
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生物信息学
蛋白别名: DKFZp762C143; E2 ubiquitin-conjugating enzyme Q2; ubiquitin carrier protein Q2; ubiquitin conjugating enzyme E2Q family member 2; ubiquitin-protein ligase Q2; unnamed protein product
UniProt ID: (Human) Q8WVN8
Entrez Gene ID: (Human) 92912
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
