Invitrogen

VAMP4 Polyclonal Antibody

Name: VAMP4 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-768

高级验证

3个已发表图片

9 篇参考文献

查看其他7个VAMP4抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

VAMP4 Antibody in Immunocytochemistry (ICC/IF)

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VAMP4 Antibody (PA1-768) in ICC/IF

Immunofluorescence analysis of VAMP4 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with VAMP4 Rabbit Polyclonal Antibody (Product # PA1-768) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

VAMP4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

VAMP4 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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VAMP4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP4 was observed in Mouse Brain, Rat Brain, Rat Lung and Rat Liver in Western Blot using VAMP4 Polyclonal Antibody (Product # PA1-768). VAMP4 is reported to be expressed in brain tissue and not in other tissues like lung and liver. {RE}

产品信息

PA1-768

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

2 µg/mL

查看4篇已发表文章 4篇已发表文章

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant rat VAMP-4 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2212790

产品详细信息

PA1-768 detects VAMP-4 from human and mouse samples.

PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining.

Immunofluorescence staining with this antibody requires saponin permeabilization.

The PA1-768 antigen is recombinant rat VAMP-4.

靶标信息

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered.

仅用于科研。不用于诊断过程。未经明确授权不得转售。