Invitrogen

VASP Recombinant Rabbit Monoclonal Antibody (SY02-16)

Name: VASP Recombinant Rabbit Monoclonal Antibody (SY02-16)
Brand: Invitrogen
SKU: MA5-32097

高级验证

查看其他37个VASP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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VASP Antibody (MA5-32097) in WB

Western blot was performed using Anti-VASP Recombinant Rabbit Monoclonal Antibody (SY02-16) (Product # MA5-32097) and a 50 kDa band corresponding to VASP was observed in HEL 92.1.7, THP-1, A431, HCT 116, and HeLa but not in HEK293 and SHSY5Y which is reported to be low to negative for VASP expression. Whole-cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), THP-1 (Lane 2), A-431 (Lane 3), HCT 116 (Lane 4), HEK-293 (Lane 5), HeLa (Lane 6), SH-SY5Y (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX)... View More

Product Image Gallery

VASP Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of VASP was observed in HEL 92.1.7, THP-1, A431, HCT 116, and HeLa but not in HEK-293 and SH-SY5Y which is reported to have low to negative expression for VASP, using Anti-VASP Recombinant Rabbit Monoclonal Antibody (SY02-16) (Product # MA5-32097) in Western Blot. {RE}

Immunogen image

Immunogen Image VASP Recombinant Rabbit Monoclonal Antibody (SY02-16)

产品信息

MA5-32097

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SY02-16

抗原

Synthetic peptide within Human VASP aa 51-100

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2809390

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

仅用于科研。不用于诊断过程。未经明确授权不得转售。