Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
VGLL2 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 2
VGLL2 Antibody (PA5-100693) in ICC/IF
Immunocytochemistry analysis of VGLL2 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with VGLL2 polyclonal antibody (Product # PA5-100693) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-100693
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human VGLL2(Accession Q8N8G2), corresponding to amino acid residues S116-H166.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total VGLL2.
靶标信息
VGLL2 is the master regulator in the wing development in Drosophila, which acts through a complex by interacting with scalloped, a homolog of vertebrate TEF proteins. Northern and dot blot analyses of human adult tissues detected VGLL2 expression in skeletal muscle. In situ hybridization revealed VGLL2 expression in differentiating somites and branchial arches during mouse embryogenesis. VITO1 is the clone of VGLL2. It contains a scalloped interaction domain (SID). VITO1 is more highly expressed in differentiated myotubes from the mouse C2C12 myogenic cell line than in undifferentiated cells. During differentiation in mouse C2C12 cell line, VGLL2 mRNA levels increase and VGLL2 protein translocates from the cytoplasm to the nucleus. Interference with VITO1 expression by small interfering RNA attenuated differentiation in C2C12 muscle cells. Therefore, this protein is an important component of the myogenic program.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Protein VITO1; RP1-179P9.2; Transcription cofactor vestigial-like protein 2; Vestigial and Tondu related protein 1; vestigial like 2; vestigial like 2 homolog; vestigial-like family member 2; Vgl-2
基因别名: C130057C21Rik; vgl-2; VGL2; VGLL2; VITO-1; VITO1
UniProt ID: (Human) Q8N8G2, (Mouse) Q8BGW8
Entrez Gene ID: (Human) 245806, (Mouse) 215031, (Rat) 309772
