Invitrogen

WDR76 Polyclonal Antibody

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)
高级验证 (1)

WDR76 Antibody in Western Blot (WB)

WDR76 Antibody in Western Blot (WB)

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WDR76 Antibody (PA5-24147) in WB

Western blot analysis of WDR76 was performed by loading 500 ng of whole cell extract from equal numbers of wild type mouse embryonic E14 stem cells (E14 mESCs) and E14 mESCs in which the WDR76 locus has been truncated via CRISPR editing. Sample was loaded in reducing sample buffer (5x = 10% SDS, 50% glycerol (v/v), 0.25% bromophenol blue (w/v) and 10 mM beta mercaptoethanol) on a 14% polyacrylamide gel. Proteins were transferred 0.22 micron PVDF membrane in Towbins buffer (25 mM Tris, 192 mM glycine, 20% methanol (v/v), 0.1% SDS (v/v) at 1... View More

WDR76 Antibody in Western Blot (WB)

WDR76 Antibody in Western Blot (WB)

WDR76 Antibody in Western Blot (WB)

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WDR76 Antibody in Western Blot (WB) — Western blot analysis of WDR76 was performed by loading 500 ng of whole cell extract from equal numbers of wild type mouse embryonic E14 stem cells (E14 mESCs) and E14 mESCs in which the WDR76 locus has been truncated via CRISPR editing. Sample was loaded in reducing sample buffer (5x = 10% SDS, 50% glycerol (v/v), 0.25% bromophenol blue (w/v) and 10 mM beta mercaptoethanol) on a 14% polyacrylamide gel. Proteins were transferred 0.22 micron PVDF membrane in Towbins buffer (25 mM Tris, 192 mM glycine, 20% methanol (v/v), 0.1% SDS (v/v) at 100 mA for 30 minutes. WDR76 was detected at approximately 70 kDa using a WDR76 polyclonal antibody (Product # PA5-24147) at a dilution of 1:1,000. This incubation proceeded overnight and was followed by incubation with a 1:10,000 dilution of a secondary HRP-conjugated goat anti mouse IgG antibody (Product # 31432) at a dilution of 1:20,000. Chemiluminescent detection was performed. The loading level of the wildtype and CRISPR targeted E14 mESCs was compared by blotting for histone H3 using a 1:20,000 dilution of this antibody and the same HRP-conjugated goat anti mouse IgG antibody as described above. The WDR76 confirmed atthe protein level that while WDR76 is detected in the wildtype E14 mESCs, the CRISPR targeted line does not contain fµll length WDR76. Data provided courtesy of Kate Malecek and Alex Ruthenburg.

WDR76 Antibody in Western Blot (WB) — Western blot analysis of WDR76 in mouse NIH-3T3 cell line lysates. Samples were incubated with WDR76 polyclonal antibody (Product # PA5-24147). Lysates: 35 µg/lane. WDR76 protein (arrow).

WDR76 Antibody in Western Blot (WB) — Western blot analysis of WDR76 in U251 cell line lysates. Samples were incubated with WDR76 polyclonal antibody (Product # PA5-24147). Lysates: 35 µg/lane. WDR76 protein (arrow).

WDR76 Antibody — The specificity of anti-WDR76 polyclonal antibody (Product # PA5-24147) was demonstrated by CRISPR targeted knockout in mouse embryonic E14 stem cells. Western blot analysis of whole cell lysates using this antibody showed no detection of WDR76 protein expression in the E14 mESCs in which the WDR76 locus has been truncated via CRISPR editing (lane 2) compared to wildtype cells (lane 1). {KO}

Immunogen image

WDR76 Polyclonal Antibody

Immunogen Image WDR76 Polyclonal Antibody

产品信息

PA5-24147

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 455-484 amino acids from the C-terminal region of human WDR76

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

PA5-24147 detects WDR76 in WB application.

靶标信息

Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: WD repeat-containing protein 76

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基因别名: 5830411K18Rik; 6030457D09; AA517836; CDW14; WDR76

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UniProt ID: (Human) Q9H967, (Mouse) A6PWY4

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Entrez Gene ID: (Human) 79968, (Mouse) 241627

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molecular_function

cellular response to DNA damage stimulus biological_process