Invitrogen

WISP1 Polyclonal Antibody

Name: WISP1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-47183
Price: 4334.00 CNY
Availability: InStock

查看其他13个WISP1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)

WISP1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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WISP1 Antibody (PA5-47183) in IHC (F)

Immunohistochemical analysis of WISP1 in immersion fixed frozen sections of mouse embryo (13 d.p.c.). Samples were incubated in WISP1 polyclonal antibody (Product # PA5-47183) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to developing epithelial cel... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image WISP1 Polyclonal Antibody

产品信息

PA5-47183

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse WISP-1/CCN4 Thr23-Asn367

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2610223

产品详细信息

In Western blots, approximately 10% cross-reactivity with recombinant human (rh) WISP-1 is observed and less than 1% cross-reactivity with rhCTGF and recombinant mouse NOV is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。