Invitrogen

YIPF4 Polyclonal Antibody

查看其他4个YIPF4抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (5)

YIPF4 Antibody in Immunocytochemistry (ICC/IF)

YIPF4 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

YIPF4 Antibody (PA5-112218) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of YIPF4 in U251MG cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with YIPF4 Polyclonal Antibody (Product # PA5-112218) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Golgi apparatus.

YIPF4 Antibody in Immunocytochemistry (ICC/IF)

YIPF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

YIPF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

YIPF4 Antibody in Western Blot (WB)

YIPF4 Antibody in Immunoprecipitation (IP)

Product Image Gallery

YIPF4 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of YIPF4 in U251MG cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with YIPF4 Polyclonal Antibody (Product # PA5-112218) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Golgi apparatus.

YIPF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of YIPF4 in formalin-fixed paraffin embedded sections human corpus uteri tissue sections using YIPF4 Polyclonal Antibody (Product # PA5-112218) at a dilution of 1:100.

YIPF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of YIPF4 in formalin-fixed paraffin embedded sections human kidney tissue sections using YIPF4 Polyclonal Antibody (Product # PA5-112218) at a dilution of 1:100.

YIPF4 Antibody in Western Blot (WB) — Western Blot analysis of YIPF4 using YIPF4 Polyclonal Antibody (Product # PA5-112218) at a dilution of 1:500. Lane A: A431 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa.

YIPF4 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of YIPF4 was performed on (Lane A) 0.5 mg A431 whole cell lysate using 4 µL of YIPF4 Polyclonal Antibody (Product # PA5-112218) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 26 kDa.

产品信息

PA5-112218

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:100-1:500

-

免疫沉淀 (IP)

1-5 µL/mg of lysate

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human YIPF4 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography, Protein A

保存液

PBS, pH 7

内含物

0.03% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

Involved in the maintenance of the Golgi structure.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Protein YIPF4; putative protein product of Nbla11189; Yip1 domain family, member 4; YIP1 family member 4

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基因别名: FinGER4; Nbla11189; YIPF4

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UniProt ID: (Human) Q9BSR8

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Entrez Gene ID: (Human) 84272

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protein binding