Rockland

ZIC-2 Polyclonal Antibody

Name: ZIC-2 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-848

查看其他11个ZIC2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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ZIC-2 Antibody (600-401-848) in WB

Western blot using Rocklands Affinity Purified anti-ZIC2 antibody shows detection of a band ~55 kDa (arrowhead) corresponding to ZIC2 in lysates from (lane 1) mouse brain and (lane 2) rat brain. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-ZIC2 antibody. Signal was detected using standard techniques. The upper band in the mouse brain lysate represents Zic2.

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Immunogen image

Immunogen Image ZIC-2 Polyclonal Antibody

产品信息

600-401-848

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500- 1:2,000

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

免疫沉淀 (IP)

1:500-1:2,000

产品规格

种属反应

Dog, Chimpanzee, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 180-210 of Mouse ZIC-2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against mouse ZIC2 protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, mouse, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with ZIC2 from chicken, zebrafish and frog as the core 16 amino acid residues within the immunogen sequence show very high degrees of homology with ZIC2 from these sources. Cross reactivity with ZIC2 homologues from other sources has not been determined.

靶标信息

ZIC2 (zinc finger protein of the cerebellum 2) is a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. In mouse, knockout of Zic1 leads to aplasia of cerebellum and skeletal abnormalities. Mutation in Zic2 and Zic3 in mouse and/or human cause holoprosencephaly and heterotaxis, respectively. Holoprosencephaly is the most common structural anomaly of the human brain.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: cerebellum zinc finger protein 2; GENA 29; kumba; odd-paired homolog; Zic family member 2 (odd-paired homolog, Drosophila); Zinc finger protein of the cerebellum 2; Zinc finger protein ZIC 2

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基因别名: HPE5; Ku; ZIC2

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UniProt ID: (Human) O95409

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Entrez Gene ID: (Dog) 485533, (Human) 7546, (Rat) 361096, (Mouse) 22772

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功能

DNA binding transcription factor activity, sequence-specific DNA binding chromatin DNA binding metal ion binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding protein binding C2H2 zinc finger transcription factor

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