Invitrogen

ZNF313 Polyclonal Antibody

查看其他9个ZNF313抗体

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抗体检测数据 (6)
高级验证 (2)

ZNF313 Antibody in Immunocytochemistry (ICC/IF)

ZNF313 Antibody in Immunocytochemistry (ICC/IF)

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ZNF313 Antibody (PA5-112261) in ICC/IF

Immunofluorescence analysis of ZNF313 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ZNF313 Polyclonal Antibody (Product # PA5-112261, 1:250) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A27034, ... View More

ZNF313 Antibody in Immunocytochemistry (ICC/IF)

ZNF313 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZNF313 Antibody in Western Blot (WB)

ZNF313 Antibody in Western Blot (WB)

ZNF313 Antibody in Western Blot (WB)

ZNF313 Antibody in Immunoprecipitation (IP)

ZNF313 Antibody

ZNF313 Antibody

Product Image Gallery

ZNF313 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ZNF313 was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ZNF313 Polyclonal Antibody (Product # PA5-112261, 1:250) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A27034, 1:2000) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

ZNF313 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ZNF313 in formalin-fixed paraffin embedded sections human liver tissue sections using ZNF313 Polyclonal Antibody (Product # PA5-112261) at a dilution of 1:100.

ZNF313 Antibody in Western Blot (WB) — Western blot was performed using ZNF313 Polyclonal Antibody (Product # PA5-112261) and a 27 kDa band corresponding to ZNF313 was observed across all cell lines and tissues tested. Whole cell extracts (30 µg lysate) of T-47D (Lane 1), Jurkat (Lane 2), Hep G2 (Lane 3), K-562 (Lane 4), MCF7 (Lane 5), HeLa (Lane 6), SK-BR-3 (Lane 7) and Mouse Testis (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

ZNF313 Antibody in Western Blot (WB) — Western Blot analysis of ZNF313 using ZNF313 Polyclonal Antibody (Product # PA5-112261) at a dilution of 1:500. Lane A: HepG2 whole cell lysate, Lane B: 293T whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

ZNF313 Antibody in Western Blot (WB) — Knockdown of ZNF313 was achieved by transfecting Jurkat with ZNF313 specific siRNAs (Silencer® select Product # S31751, S31752). Western blot analysis (Fig. a) was performed using whole cell extracts from the ZNF313 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ZNF313 Polyclonal Antibody (Product # PA5-112261, 1:2000) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) and detected by chemiluminescence using the iBright™ FL1500 Imaging System (Product # A44115). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ZNF313.

ZNF313 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of ZNF313 was performed on (Lane A) 0.5 mg 293T whole cell lysate using 4 µL of ZNF313 Polyclonal Antibody (Product # PA5-112261) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.

ZNF313 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Jurkat cells were transfected with ZNF313 siRNA and decrease in signal intensity was observed in Western Blot application using ZNF313 Polyclonal Antibody (Product # PA5-112261). {KD}

ZNF313 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across multiple tissues owing to their inherent genetic constitution. Relative expression of ZNF313 was observed in Mouse and Rat testis as compared to Mouse and Rat brain and placenta using ZNF313 Polyclonal Antibody (Product # PA5-112261) in Western Blot (doi: 10.1093/hmg/ddn091). {RE}

产品信息

PA5-112261

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:250

-

免疫沉淀 (IP)

0.5-2 µL/mg lysate

-

产品规格

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human RNF114 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

靶标信息

ZNF313 is a novel human zinc finger protein gene that contains both ring finger and C(2)H(2) domain. It may play a role in spermatogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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