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Invitrogen
ZO-1 Monoclonal Antibody (R26.4C), eBioscience™
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图: 1 / 6
ZO-1 Antibody (14-9776-95) in ICC/IF
Immunocytochemistry of formaldehyde-fixed, permeabilized MDCK cells stained with 10 µg/mL of Anti-ZO-1 Purified, followed by 10 µg/mL of Anti-Rat IgG TRITC (left).Immunohistochemistry of acetone-fixed mouse testes stained with 10 µg/mL of Anti-ZO-1 Purified, followed by 10 µg/mL of Anti-Rat IgG TRITC (right).Nuclei are stained with DAPI.
产品信息
14-9776-95
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Human,
Mouse,
Xenopus
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
R26.4C
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
4°C
运输条件
Wet ice
RRID
产品详细信息
Description: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine. ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain. ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer. ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C. ZO-1 is thought to anchor the actin cytoskeleton to the tight junction. ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression.
Applications Reported: This R26.4C antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
