Invitrogen

ZW10 Polyclonal Antibody

查看其他7个ZW10抗体

抗体检测数据 (6)

ZW10 Antibody in Immunocytochemistry (ICC/IF)

ZW10 Antibody in Immunocytochemistry (ICC/IF)

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ZW10 Antibody (PA5-118376) in ICC/IF

Immunofluorescence staining of ZW10 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ZW10 Polyclonal Antibody (Product # PA5-118376, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

ZW10 Antibody in Immunocytochemistry (ICC/IF)

ZW10 Antibody in Immunocytochemistry (ICC/IF)

ZW10 Antibody in Western Blot (WB)

ZW10 Antibody in Western Blot (WB)

ZW10 Antibody in Immunoprecipitation (IP)

ZW10 Antibody in Immunoprecipitation (IP)

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ZW10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of ZW10 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ZW10 Polyclonal Antibody (Product # PA5-118376, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

ZW10 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of ZW10 in U2OS cells. Samples were treated with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-118376) with a dilution of 1: 100 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).

ZW10 Antibody in Western Blot (WB) — Western Blot using ZW10 Polyclonal Antibody (Product # PA5-118376) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 88 kDa. Observed band size: 90 kDa. (We are unsure of the identity of these extra bands).

ZW10 Antibody in Western Blot (WB) — Western Blot of ZW10 in Lane A: Jurkat Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118376) with a dilution of 1:500, followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 88 kDa, Observed band size: 90 kDa.

ZW10 Antibody in Immunoprecipitation (IP) — ZW10 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with ZW10 Polyclonal Antibody (Product # PA5-118376) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: ZW10 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 88 kDa. Observed band size: 90 kDa.

ZW10 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of ZW10 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118376) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 88 kDa , Observed band size : 90 kDa .

产品信息

PA5-118376

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

免疫沉淀 (IP)

5-10 µL/mg of lysate

-

产品规格

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived Human ZW10 fragment

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

靶标信息

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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