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aadA1/Aminoglycoside adenyltransferase (chloroplast transformation marker) Polyclonal Antibody
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aadA1/Aminoglycoside adenyltransferase (chloroplast transformation marker) Antibody (AS09-580) in WB
Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 µg of chlorophyll) and soluble extracts of Nicotiana tabacum leaves (3,4) were separated on a 15% acrylamide gel containing 8 M urea, transferred on nitrocellulose membrane, incubated with anti-aad 1 antibodies in 1:5,000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbit Ig G antibody in 1:10,000 dilution) and re... View More
产品信息
AS09-580
产品规格
种属反应
Algae,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from known aadA1 protein sequences.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
靶标信息
Aminoglycoside adenyltransferase (AadA; aminoglycoside O-nucleotidyltransferase) is a bacterial cytosolic enzyme typically encoded by horizontally mobile antibiotic-resistance determinants (commonly plasmid- and integron-associated) and produced as a soluble monomeric protein. Structurally, AadA adopts a two-domain architecture comprising an N-terminal nucleotidyltransferase (NTase) catalytic domain and a C-terminal alpha-helical bundle domain; the active site lies in an interdomain cleft and undergoes ligand-coupled conformational rearrangements, with ATP binding occurring prior to aminoglycoside binding to properly position the domains for catalysis. Functionally, AadA mediates aminoglycoside resistance by catalyzing a Mg2+-dependent transfer of AMP from ATP onto hydroxyl groups of target antibiotics, including O-adenylation at the 3''-hydroxyl of streptomycin and the 9-hydroxyl of spectinomycin, thereby reducing drug affinity for the ribosome and diminishing inhibition of protein synthesis; mutational and structural studies support a key role for a conserved glutamate (e.g., Glu87 in Salmonella enterica AadA) as the catalytic base and identify basic residues important for ATP coordination during the adenylation reaction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: aadA; aadA1; aadA10; aadA14; aadA2; aadA21; aadA23; aadA4; aadA5; aadA6; aadA7; aadA8; aadA9
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