MUbio

actin alpha-muscle Monoclonal Antibody (HHF35)

Name: actin alpha-muscle Monoclonal Antibody (HHF35)
Brand: MUbio
SKU: MUB0107P

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB0107P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Chicken, Human, Non-human primate, Pig, Rabbit, Rat, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

HHF35

抗原

actin alpha-muscle

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

HHF35 reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections.

The epitope recognized by α-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin gamma 1 is a cytoplasmic actin found in nonmuscle cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: actin gamma; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma, cytoplasmic 1; Atcb; cytoplasmic 2; cytoplasmic ACTG1; cytoplasmic type 5; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma 1 propeptide; Gamma-actin; type 5

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基因别名: ACT; ACTG; ACTG1; actin; BRWS2; DFNA20; DFNA26; HEL-176

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UniProt ID: (Chicken) P53478, (Human) P63261, (Rat) P63259

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Entrez Gene ID: (Pig) 397653, (Chicken) 415296, (Human) 71, (Rat) 287876

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