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alpha Actinin 2 Antibody (710947) in ICC/IF
For immunofluorescence analysis, human skeletal muscle myoblast cells were fixed and permeabilized for detection of endogenous sarcomeric alpha actin using Anti-Sarcomeric alpha Actin/ alpha Actinin 2 Recombinant Rabbit Superclonal™ Antibody (Product # 710947, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). ... View More
产品信息
710947
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
抗原
Peptides corresponding to human Sarcomeric alpha Actin/ alpha Actinin 2 [1) aa834-aa852, 2)aa5-aa24]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Monkey, Pig, Rat, Mouse
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: actinin, alpha 2; alpha-actinin skeletal muscle; Alpha-actinin skeletal muscle isoform 2; Alpha-actinin-2; F-actin cross-linking protein
基因别名: 1110008F24Rik; ACTN2; CMD1AA; CMH23
UniProt ID: (Human) P35609, (Mouse) Q9JI91
Entrez Gene ID: (Human) 88, (Mouse) 11472, (Rat) 291245
Ras guanyl-nucleotide exchange factor activity
integrin binding
calcium ion binding
protein binding
phosphatidylinositol-4,5-bisphosphate binding
cytoskeletal protein binding
structural constituent of muscle
protein domain specific binding
LIM domain binding
thyroid hormone receptor coactivator activity
titin binding
identical protein binding
protein homodimerization activity
ion channel binding
protein dimerization activity
actin filament binding
FATZ binding
titin Z domain binding
actin binding
ligand-dependent nuclear receptor transcription coactivator activity
protein binding, bridging
metal ion binding
MAPK cascade
platelet degranulation
cell adhesion
microspike assembly
muscle filament sliding
regulation of membrane potential
regulation of apoptotic process
negative regulation of potassium ion transport
positive regulation of potassium ion transport
positive regulation of GTPase activity
sarcomere organization
focal adhesion assembly
protein homotetramerization
actin filament uncapping
cardiac muscle cell development
phospholipase C-activating angiotensin-activated signaling pathway
establishment of protein localization to plasma membrane
negative regulation of potassium ion transmembrane transporter activity
positive regulation of potassium ion transmembrane transporter activity
regulation of nucleic acid-templated transcription
negative regulation of protein localization to cell surface
positive regulation of receptor activity
positive regulation of endocytic recycling
positive regulation of cation channel activity
muscle contraction
