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Invitrogen
cGMP Recombinant Rabbit Monoclonal Antibody (RM467)
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cGMP Antibody (MA5-44557) in ELISA
Competitive ELISA detecting cGMP in HeLa cells. The 96-well plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/mL of cGMP Recombinant Monoclonal antibody (Product # MA5-44557) (50 µl/well) was added and incubated. After wash and block, HeLa cell lysate (~105 cell/mL) or control (lysate buffer only) (25 µl/well) were added along with 25 µl/well of 1/5,000 diluted HRP conjugated cGMP. TMB was used to develop the color after incubation and wash.
产品信息
MA5-44557
产品规格
种属反应
Chemical
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
RM467
抗原
KLH-conjugated cGMP.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1% BSA, 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody reacts to the methoxy group of Polyethylene glycol (PEG). It is specific for methoxypoly(ethylene glycol).
靶标信息
Guanosine cyclic 3',5'-(hydrogen phosphate) is a guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. Cyclic gmp is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. [National Center for Biotechnology Information. PubChem Compound Database; CID=24316].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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