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Agrisera
eEF1B-alpha1 and 2/elongation factor 1B-alpha 1 and 1B-alpha 2 Polyclonal Antibody
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eEF1B-alpha1 and 2/elongation factor 1B-alpha 1 and 1B-alpha 2 Antibody (AS10-678) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked followi... View More
产品信息
AS10-678
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
EF1B1, also called elongation factor 1 beta 1, is a cytosolic guanine nucleotide exchange factor that reactivates EF1A by catalyzing the exchange of GDP for GTP, sustaining the delivery of aminoacyl tRNAs to ribosomes during translation elongation in plants. EF1B1 typically forms a complex with EF1B gamma and EF1B delta subunits, providing stability and regulation of exchange activity. Beyond its core role in translation, EF1B1 contributes to stress responses and developmental regulation by influencing protein synthesis capacity, and its expression adjusts with growth, hormone signaling, and environmental conditions. Because of its involvement in fundamental cellular processes, EF1B1 levels can serve as indicators of translational activity, but they may vary under stress, so careful validation is needed when using EF1B1 as a reference in expression studies.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EEF1B1; EF1B
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