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Invitrogen
mCherry Polyclonal Antibody
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mCherry Antibody (PA5-34974) in ICC/IF
mCherry Polyclonal Antibody detects mCherry protein at mCherry fusion protein by immunofluorescent analysis. Sample: mCherry fusion protein-transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 30 min, then Triton X-100 for 1 min. Green: mCherry protein stained by mCherry Polyclonal Antibody (Product # PA5-34974) diluted at 1:500. Red: mCherry is expressed in the transfected cell . Blue: Hoechst 33342 staining.
产品信息
PA5-34974
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
已发表种属
C. elegans,
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Full length mCherry recombinant protein.
偶联物
形式
Liquid
浓度
0.22 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T whole cell extract, human PLK4-mCherry-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
