Invitrogen

nNOS Polyclonal Antibody

6个已发表图片
18 篇参考文献
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说明书
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nNOS Polyclonal Antibody

产品信息

PA1-033

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:100-1:1,000
查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

-
查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20-1:200
查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:500
查看3篇已发表文章 3篇已发表文章
产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325021

产品详细信息

PA1-033 detects nNOS from human, rat and mouse samples.

PA1-033 has been successfully used in Western blot, ICC/IF and immunohistochemisty (paraffin) procedures. By Western blot, this antibody detects an ~160 kDa protein. Detects a few non-specific bands in Western blot analysis of rat and mouse brain lysates. This antibody is not recommended for MCF-7, PC-12, SK-N-C or U87-MG cells in Western blot applications.

The PA1-033 immunogen is a synthetic peptide to residues C N(1411) R L R S E S I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, mouse, frog, and rabbit nNos proteins. This peptide (Cat. # PEP-190) is available for use in neutralization and control experiments.

靶标信息

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

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基因别名: 2310005C01Rik; Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; NOS1

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UniProt ID: (Human) P29475, (Rat) P29476, (Mouse) Q9Z0J4

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Entrez Gene ID: (Human) 4842, (Rat) 24598, (Mouse) 18125

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功能
nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding sodium channel regulator activity enzyme binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding NADPH-hemoprotein reductase activity amino acid binding ATPase binding oxidoreductase
参与通路
response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction nitric oxide mediated signal transduction myoblast fusion female pregnancy aging negative regulation of cell proliferation response to heat response to lead ion negative regulation of calcium ion transport into cytosol response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine neurotransmitter biosynthetic process exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen cell redox homeostasis response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine regulation of neurogenesis negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation negative regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of cardiac conduction muscle contraction regulation of heart contraction response to organonitrogen compound response to organic cyclic compound response to nutrient levels negative regulation of peptidyl-serine phosphorylation cellular response to epinephrine stimulus
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