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Invitrogen
Androstenedione Competitive ELISA Kit
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产品规格
检测灵敏度
2.3 pg/mL
测定范围
5-1,200 pg/mL
检测样本类型/体积
{Saliva=50 µL, Urine=50 µL, Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
Androstenedione
蛋白家族
Chromone
均相（无需洗涤）
Chemical
检测试剂盒格式
Competitive ELISA
检测抗体结合物
HRP
标签或染料
Unconjugated
关于本试剂盒
The multispecies Androstenedione ELISA quantitates androstenedione in serum, plasma, and saliva, or urine.
原理方法:
The Androstenedione Competitive ELISA research-use-only kit is designed to quantitatively measure androstenedione independent of species. An adrostenedione standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with donkey anti-sheep IgG antibodies. Rabbit polyclonal antibodies and androstenedione-peroxidase conjugate is added to the wells. The androstenedione conjugate and any androstenedione in the sample will compete to bind to the rabbit androstenedione antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound testosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of androstenedione present in the sample.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Androstenedione is a steroid hormone also known as androst-4-ene-3,17-dione. It is synthesized from dehydroepiandrosterone (DHEA) or 17-hydroxyprogesterone. By itself, androstenedione is a weak androgen though it is a precursor for biosynthesis of stronger androgens, such as testosterone and estrogens. Therefore, androstenedione is classified as a controlled drug and a supplement to enhance athletic performance and body energy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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